Rabbit polyclonal antibody to PAK1 (Phospho-Thr212)
Specificity
The antibody detects endogenous level of PAK1 only when phosphorylated at threonine 212.
Applications
WB, IHC, ICC/IF
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around phosphorylation site of threonine 212 (P-V-T(p)-P-T) derived from Human PAK1.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1.0 mg/mL
Formulation
Supplied in phosphate-buffered saline (without Mg²? and Ca²?), pH 7.4, containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
Alpha-PAK, p21-activated kinase 1, p65-PAK, PAK-1, Serine/threonine-protein kinase PAK 1
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PAK1 (Phospho-Thr212) Antibody #11154 (left) or the same antibody preincubated with blocking peptide (right).
