Anti-PI 3 Kinase p85 alpha Antibody (A12507)

Name: Anti-PI 3 Kinase p85 alpha Antibody
Brand: Antibodies.com
SKU: A12507

Enlarge Image

This product is discontinued

Anti-PI 3 Kinase p85 alpha Antibody (A12507) has been discontinued and is no longer available.

View all PI 3 Kinase p85 alpha Antibodies.

Specifications

Name

Anti-PI 3 Kinase p85 alpha Antibody

Description

Rabbit polyclonal antibody to PI 3 Kinase p85 alpha.

Applications

WB, IHC

Dilutions

WB: 1:1,000-1:5,000, IHC: 1:50-1:200

Reactivity

Human, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 125-320 of human PI3 Kinase p85 alpha (NP_852664.1).

Sequence

PDIAPPLLIKLVEAIEKKGLECSTLYRTQSSSNLAELRQLLDCDTPSVDLEMIDVHVLADAFKRYLLDLPNPVIPAAVYSEMISLAPEVQSSEEYIQLLKKLIRSPSIPHQYWLTLQYLLKHFFKLSQTSSKNLLNARVLSEIFSPMLFRFSAASSDNTENLIKVIEILISTEWNERQPAPALPPKPPKPTTVANN

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

85 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (2)

Enlarge Image

Western Blot - Anti-PI 3 Kinase p85 alpha Antibody (A12507)

Western blot analysis of extracts of U-87MG cells, using Anti-PI 3 Kinase p85 alpha Antibody (A12507) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

Enlarge Image

Immunohistochemistry - Anti-PI 3 Kinase p85 alpha Antibody (A12507)

Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-PI 3 Kinase p85 alpha Antibody (A12507) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.