Rabbit polyclonal antibody to PKCd (Phospho-Ser645)
Specificity
The antibody detects endogenous level of PKCd only when phosphorylated at serine 645.
Applications
WB, IHC
Reactivity
Human, Rat
Immunogen
Peptide sequence around phosphorylation site of serine 645 (R-L-S(p)-Y-S) derived from Human PKCd.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1.0 mg/mL
Formulation
Supplied in phosphate-buffered saline (without Mg²? and Ca²?), pH 7.4, containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
EC:2.7.10.2, nPKC-delta, PRKCD, Protein kinase C delta type, Tyrosine-protein kinase PRKCD
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PKCd (Phospho-Ser645) Antibody #11296 (left) or the same antibody preincubated with blocking peptide (right).
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