Rabbit polyclonal antibody to Shc1 (Phospho-Tyr427)
Specificity
The antibody detects endogenous level of Shc1 only when phosphorylated at tyrosine 427.
Applications
WB, IHC, ICC/IF
Reactivity
Human
Immunogen
Peptide sequence around phosphorylation site of tyrosine 427 (P-S-Y(p)-V-N derived from Human Shc1.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1.0 mg/mL
Formulation
Supplied in phosphate-buffered saline (without Mg²? and Ca²?), pH 7.4, containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
SH2 domain protein C1, SHC-transforming protein 1, SHC-transforming protein 3, SHC-transforming protein A, SHCA, Src homology 2 domain-containing-transforming protein C1
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Shc1 (Phospho-Tyr427) Antibody #11317 (left) or the same antibody preincubated with blocking peptide (right).
