Rabbit polyclonal antibody to Synaptotagmin 1 (Phospho-Ser309)
Specificity
The antibody detects endogenous level of Synaptotagmin 1 only when phosphorylated at serine 309.
Applications
ICC/IF
Dilutions
Immunofluorescence: 1:100~1:200
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around phosphorylation site of serine 309 (G-L-S(p)-D-P) derived from Human Synaptotagmin 1.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1.0 mg/mL
Predicted MW
46kd
Formulation
Supplied in phosphate-buffered saline (without Mg²? and Ca²?), pH 7.4, containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
p65, SVP65, Synaptotagmin I, Synaptotagmin-1, SYT, SYT1, SytI