Anti-Collagen I Antibody [ARC53610] (A305981)

Name: Anti-Collagen I Antibody [ARC53610]
Brand: Antibodies.com
SKU: A305981

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This product is discontinued

Anti-Collagen I Antibody [ARC53610] (A305981) has been discontinued and is no longer available.

View all Anti-Collagen I Antibodies.

Specifications

Name

Anti-Collagen I Antibody [ARC53610]

Description

Rabbit monoclonal [ARC53610] antibody to Collagen I.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:1,000-1:5,000, IHC: 1:100-1:500, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1100-1200 of human Collagen I/COL1A1 (NP_000079.2).

Sequence

GEQGDRGIKGHRGFSGLQGPPGPPGSPGEQGPSGASGPAGPRGPPGSAGAPGKDGLNGLPGPIGPPGPRGRTGDAGPVGPPGPPGPPGPPGPPSAGFDFSF

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC53610

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

220 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Alpha 1 type I collagen, Alpha 2 type I collagen, alpha 2 type I procollagen, alpha 2(I) procollagen, alpha 2(I)-collagen, Alpha-1 type I collagen, alpha1(I) procollagen, CO1A1_HUMAN, COL1A1, COL1A2, collagen alpha 1 chain type I, Collagen alpha-1(I) chain, collagen alpha-1(I) chain preproprotein, Collagen I alpha 1 polypeptide, Collagen I alpha 2 polypeptide, collagen of skin, tendon and bone, alpha-1 chain, collagen of skin, tendon and bone, alpha-2 chain, Collagen type I alpha 1, Collagen type I alpha 2, EDSC, OI1, OI2, OI3, OI4, pro-alpha-1 collagen type 1, type I proalpha 1, Type I procollagen, type I procollagen alpha 1 chain

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-Collagen I Antibody [ARC53610] (A305981)

Western blot analysis of extracts of NIH/3T3 cells, using Anti-Collagen I Antibody [ARC53610] (A305981) at 1:2,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Figure 2: Immunohistochemistry - Anti-Collagen I Antibody [ARC53610] (A305981)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-Collagen I Antibody [ARC53610] (A305981) at a dilution of 1:500(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-Collagen I Antibody [ARC53610] (A305981)

Immunohistochemistry analysis of paraffin-embedded mouse lung using Anti-Collagen I Antibody [ARC53610] (A305981) at a dilution of 1:500(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-Collagen I Antibody [ARC53610] (A305981)

Immunohistochemistry analysis of paraffin-embedded rat kidney using Anti-Collagen I Antibody [ARC53610] (A305981) at a dilution of 1:500(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.