Anti-Cyclin E1 Antibody [ARC51383 + ARC51384] (A305271)

Name: Anti-Cyclin E1 Antibody [ARC51383 + ARC51384]
Brand: Antibodies.com
SKU: A305271

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This product is discontinued

Anti-Cyclin E1 Antibody [ARC51383 + ARC51384] (A305271) has been discontinued and is no longer available.

View all Anti-Cyclin E1 Antibodies.

Specifications

Name

Anti-Cyclin E1 Antibody [ARC51383 + ARC51384]

Description

Rabbit Oligoclonal [ARC51383 + ARC51384] antibody to Cyclin E1.

Applications

WB, IHC

Dilutions

WB: 1:2,000-1:10,000, IHC: 1:50-1:200

Reactivity

Human, Mouse

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 300-400 of human Cyclin E1 Rabbit PolymAb® (NP_001229.1).

Sequence

AASALYHFSSSELMQKVSGYQWCDIENCVKWMVPFAMVIRETGSSKLKHFRGVADEDAHNIQTHRDSLDLLDKARAKKAMLSEQNRASPLPSGLLTPPQSG

Host

Rabbit

Clonality

Oligoclonal

Clone ID

ARC51383 + ARC51384

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

47 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

CCNE, Ccne1, CCNE1_HUMAN, cyclin E variant ex5del, cyclin E variant ex7del, Cyclin Es, Cyclin Et, CyclinE, G1/S specific cyclin E, G1/S-specific cyclin-E1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (2)

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Figure 1: Western Blot - Anti-Cyclin E1 Antibody [ARC51383 + ARC51384] (A305271)

Western blot analysis of K-562, using Anti-Cyclin E1 Antibody [ARC51383 + ARC51384] (A305271) at 1:10,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.

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Figure 2: Immunohistochemistry - Anti-Cyclin E1 Antibody [ARC51383 + ARC51384] (A305271)

Immunohistochemistry analysis of paraffin-embedded human thyroid cancer using Anti-Cyclin E1 Antibody [ARC51383 + ARC51384] (A305271) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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