Anti-EGFR Antibody (A80545)

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This product is discontinued

Anti-EGFR Antibody (A80545) has been discontinued and is no longer available.

View all EGFR Antibodies.

Specifications

Name

Anti-EGFR Antibody

Description

Rabbit polyclonal antibody to EGFR.

Applications

WB, IHC

Dilutions

WB: 1:500-1:1000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1100-1200 of human EGFR (NP_005219.2).

Sequence

RPAGSVQNPVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQISLDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRV

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

180kDa

Formulation

Supplied in Phosphate Buffered Saline, pH 7.30, with 0.02% Sodium Azide and 50% Glycerol.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Epidermal growth factor receptor, ERBB, ERBB1, HER1, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-EGFR Antibody (A80545)

Western blot analysis of extracts of various cell lines, using Anti-EGFR Antibody (A11576) at 1:1,000 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) (HRP) (AS014) at 1:10,000 dilution. Lysates / proteins: 25µg per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

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Immunohistochemistry - Anti-EGFR Antibody (A80545)

Immunohistochemistry of paraffin-embedded mouse liver using Anti-EGFR Antibody (A11576) at dilution of 1:100 (40x lens).

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Immunohistochemistry - Anti-EGFR Antibody (A80545)

Immunohistochemistry of paraffin-embedded mouse kidney using Anti-EGFR Antibody (A11576) at dilution of 1:100 (40x lens).

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