Anti-Ezrin Antibody (A12693)

Name: Anti-Ezrin Antibody
Brand: Antibodies.com
SKU: A12693
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to Ezrin for WB, ICC/IF and IP.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-Ezrin Antibody

Description

Rabbit polyclonal antibody to Ezrin.

Applications

WB, ICC/IF, IP

Dilutions

WB: 1:500-1:2,000, ICC/IF: 1:50-1:100, IP: 1:50-1:100

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 350-470 of human Ezrinin (NP_003370.2).

Sequence

RLQDYEEKTKKAERELSEQIQRALQLEEERKRAQEEAERLEADRMAALRAKEELERQAVDQIKSQEQLAAELAEYTAKIALLEEARRRKEDEVEEWQHRAKEAQDDLVKTKEELHLVMTAP

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

90 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

CVIL, CVL, Cytovillin, Cytovillin 2, DKFZp762H157, Epididymis secretory protein Li 105, EZR, EZRI_HUMAN, FLJ26216, HEL S 105, MGC1584, p81, VIL 2, VIL2, Villin 2, Villin 2 (ezrin), Villin 2 ezrin, Villin-2, Villin2

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-Ezrin Antibody (A12693)

Western blot analysis of extracts of various cell lines, using Anti-Ezrin Antibody (A12693) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 2: Immunofluorescence - Anti-Ezrin Antibody (A12693)

Immunofluorescence analysis of C6 cells using Anti-Ezrin Antibody (A12693) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).

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Figure 3: Immunofluorescence - Anti-Ezrin Antibody (A12693)

Immunofluorescence analysis of U-2 OS cells using Anti-Ezrin Antibody (A12693) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).

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Figure 4: Western Blot - Anti-Ezrin Antibody (A12693)

Immunoprecipitation analysis of 200µg extracts of 293T cells using 3µg of Anti-Ezrin Antibody (A12693). This Western blot was performed on the immunoprecipitate using Anti-Ezrin Antibody (A12693) at a dilution of 1:1000.

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