Anti-FKHRL1 (phospho Ser253) Antibody (A93680)

Name: Anti-FKHRL1 (phospho Ser253) Antibody
Brand: Antibodies.com
SKU: A93680
Price: 185.0 USD
Availability: InStock

This product is identical to Novus Biologicals (NB100-81929). Discover more for less.

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$185

Rabbit polyclonal antibody to FKHRL1 (phospho Ser253) for WB, IHC and ELISA.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Specifications

Name

Anti-FKHRL1 (phospho Ser253) Antibody

Description

Rabbit polyclonal antibody to FKHRL1 (phospho Ser253).

Specificity

This antibody detects endogenous levels of FKHRL1 only when phosphorylated at Ser253.

Applications

WB, IHC, ELISA

Dilutions

WB: 1:500-1:1000, IHC: 1:50-1:100, ELISA: 1:1000

Reactivity

Human, Mouse

Immunogen

Synthetic peptide derived from human FKHRL1 around the phosphorylation site of Ser253 (amino acids 220-269).

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.

Molecular Weight

71kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

AF6q21, AF6q21 protein, DKFZp781A0677, FKHR2, FKHRL 1, FKHRL1, FKHRL1P2, Forkhead (Drosophila) homolog (rhabdomyosarcoma) like 1, Forkhead box O3, Forkhead box O3A, Forkhead box protein O3, Forkhead box protein O3A, Forkhead Drosophila homolog of in rhabdomyosarcoma like 1, Forkhead homolog (rhabdomyosarcoma) like 1, Forkhead in rhabdomyosarcoma like 1, Forkhead in rhabdomyosarcoma-like 1, FOX O3A, FOXO2, foxo3, FOXO3_HUMAN, MGC12739, MGC31925

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-FKHRL1 (phospho Ser253) Antibody (A93680)

Western blot analysis of lysates from LOVO cells treated with serum 20% 30' using Anti-FKHRL1 (phospho Ser253) Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.

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Figure 2: Immunohistochemistry - Anti-FKHRL1 (phospho Ser253) Antibody (A93680)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Anti-FKHRL1 (phospho Ser253) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.

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Figure 3: Western Blot - Anti-FKHRL1 (phospho Ser253) Antibody (A93680)

Western blot analysis of HELA cells using Anti-FKHRL1 (phospho Ser253) Antibody.

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Figure 4: Immunohistochemistry - Anti-FKHRL1 (phospho Ser253) Antibody (A93680)

Immunohistochemical analysis of paraffin-embedded human colon tissue using Anti-FKHRL1 (phospho Ser253) Antibody at 1:200 (4°C overnight). Negative control was secondary antibody only.