Anti-HDAC1 Antibody [AMC0232] (A308654)

Name: Anti-HDAC1 Antibody [AMC0232]
Brand: Antibodies.com
SKU: A308654

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This product is discontinued

Anti-HDAC1 Antibody [AMC0232] (A308654) has been discontinued and is no longer available.

View all Anti-HDAC1 Antibodies.

Specifications

Name

Anti-HDAC1 Antibody [AMC0232]

Description

Mouse monoclonal [AMC0232] antibody to HDAC1.

Applications

WB, IHC, IP, ChIP

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ChIP: 1:50-1:200

Reactivity

Human, Mouse

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 393-482 of human HDAC1 (Q13547).

Sequence

SGDEDEDDPDKRISICSSDKRIACEEEFSDSEEEGEGGRKNSSNFKKAKRVKTEDEKEKDPEEKKEVTEEEKTKEEKPEAKGVKEEVKLA

Host

Mouse

Clonality

Monoclonal

Clone ID

AMC0232

Isotype

IgG1

Light Chains

kappa

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

62 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

DKFZp686H12203, GON 10, HD1, HDAC 1, HDAC1_HUMAN, Histone deacetylase 1, Reduced potassium dependency yeast homolog like 1, RPD3, RPD3L1

Isotype Controls

Suitable Secondaries

See all Anti-Mouse IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (2)

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Figure 1: Western Blot - Anti-HDAC1 Antibody [AMC0232] (A308654)

Western blot analysis of extracts of various cell lines, using Anti-HDAC1 Antibody [AMC0232] (A308654) at 1:1,000 dilution. The secondary antibody was HRP Goat Anti-Mouse IgG (H+L) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 2: Immunohistochemistry - Anti-HDAC1 Antibody [AMC0232] (A308654)

Immunohistochemistry analysis of paraffin-embedded human esophageal using Anti-HDAC1 Antibody [AMC0232] (A308654) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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