Anti-Histone H2A (acetyl Lys5) Antibody (A91516)

Name: Anti-Histone H2A (acetyl Lys5) Antibody
Brand: Antibodies.com
SKU: A91516
Price: 435.0 USD
Availability: InStock

Enlarge Image

$435

Rabbit polyclonal antibody to Histone H2A (acetyl Lys5) for WB and ICC/IF.

100% Guarantee

Price Match Guarantee

Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

Contact Us

Telephone

+1 (314) 370-6046

orders@antibodies.com

Guarantees

100% Quality Guarantee

Price Match Guarantee

Tags:

Nuclear Marker

Specifications

Name

Anti-Histone H2A (acetyl Lys5) Antibody

Description

Rabbit polyclonal antibody to Histone H2A (acetyl Lys5).

Applications

WB, ICC/IF

Dilutions

WB: 1:500-1:1,000, ICC/IF: 1:50-1:100

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic acetylated peptide around K5 of human Histone H2A (NP_003508.1).

Sequence

GRGKQ

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

14 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

H2A, H2a 615, H2A GL101, H2A histone family member A, H2A histone family member M, H2A.1, H2A.2, H2A/a, H2A/m, H2A/O, H2A/q, H2A1, H2A1B_HUMAN, H2A2, H2AFA, H2AFE, H2AFL, H2AFM, H2AFO, H2AFQ, HIST1H2A, HIST1H2AB, HIST1H2AE, HIST1H2AJ, HIST2H2AA, HIST2H2AA3, HIST2H2AB, HIST2H2AC, Histone 1 H2ab, Histone 1 H2ae, Histone 2 H2aa3, Histone 2 H2ab, Histone 2 H2ac, Histone cluster 1 H2ab, Histone cluster 1 H2ae, Histone H2A type 1 B, Histone H2A type 1 C, Histone H2A type 1 E, Histone H2A type 1 J, Histone H2A type 1-B/E, Histone H2A-alpha, Histone H2A-beta, Histone H2A.1, Histone H2A.2, Histone H2A/a, Histone H2A/m, Histone H2AE, Hta 1, HTA 2, Hta1, Hta1p, HTA2, Hta2p, MGC74460, SPT 11, SPT11

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

Enlarge Image

Figure 1: Western Blot - Anti-Histone H2A (acetyl Lys5) Antibody (A91516)

Western blot analysis of extracts of various cell lines, using Anti-Histone H2A (acetyl Lys5) Antibody (A91516) at 1:1,000 dilution. C2C12 cells and C6 cells were treated by TSA (1 uM) at 37°C for 18 hours. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

Enlarge Image

Figure 2: Immunofluorescence - Anti-Histone H2A (acetyl Lys5) Antibody (A91516)

Immunofluorescence analysis of C6 cells using Anti-Histone H2A (acetyl Lys5) Antibody (A91516) at a dilution of 1:100. C6 cells were treated by TSA (1 uM) at 37°C for 18 hours. DAPI was used to stain the cell nuclei (blue).

Enlarge Image

Figure 3: Immunofluorescence - Anti-Histone H2A (acetyl Lys5) Antibody (A91516)

Immunofluorescence analysis of HeLa cells using Anti-Histone H2A (acetyl Lys5) Antibody (A91516) at a dilution of 1:100. HeLa cells were treated by TSA (1 uM) at 37°C for 18 hours. DAPI was used to stain the cell nuclei (blue).

Publishing research using Anti-Histone H2A (acetyl Lys5) Antibody (A91516)? Please let us know so that we can list the citation on this page.