Anti-Histone H2A Antibody [ARC2072] (A307778)

Name: Anti-Histone H2A Antibody [ARC2072]
Brand: Antibodies.com
SKU: A307778
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC2072] antibody to Histone H2A for WB, IHC and IP.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Tags:

Nuclear Marker

Specifications

Name

Anti-Histone H2A Antibody [ARC2072]

Description

Rabbit monoclonal [ARC2072] antibody to Histone H2A.

Applications

WB, IHC, IP

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, IP: 1:500-1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Histone H2A (P04908).

Sequence

MSGRGKQGGKARAKAKTRSSRAGLQFPVGRVHRLLRKGNYSERVGAGAPVYLAAVLEYLTAEILELAGNAARDNKKTRIIPRHLQLAIRNDEELNKLLGR

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC2072

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

14 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

H2A, H2a 615, H2A GL101, H2A histone family member A, H2A histone family member M, H2A.1, H2A.2, H2A/a, H2A/m, H2A/O, H2A/q, H2A1, H2A1B_HUMAN, H2A2, H2AFA, H2AFE, H2AFL, H2AFM, H2AFO, H2AFQ, HIST1H2A, HIST1H2AB, HIST1H2AE, HIST1H2AJ, HIST2H2AA, HIST2H2AA3, HIST2H2AB, HIST2H2AC, Histone 1 H2ab, Histone 1 H2ae, Histone 2 H2aa3, Histone 2 H2ab, Histone 2 H2ac, Histone cluster 1 H2ab, Histone cluster 1 H2ae, Histone H2A type 1 B, Histone H2A type 1 C, Histone H2A type 1 E, Histone H2A type 1 J, Histone H2A type 1-B/E, Histone H2A-alpha, Histone H2A-beta, Histone H2A.1, Histone H2A.2, Histone H2A/a, Histone H2A/m, Histone H2AE, Hta 1, HTA 2, Hta1, Hta1p, HTA2, Hta2p, MGC74460, SPT 11, SPT11

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (6)

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Figure 1: Western Blot - Anti-Histone H2A Antibody [ARC2072] (A307778)

Western blot analysis of extracts of various cell lines, using Anti-Histone H2A Antibody [ARC2072] (A307778) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 2: Western Blot - Anti-Histone H2A Antibody [ARC2072] (A307778)

Western blot analysis of extracts of Rat liver, using Anti-Histone H2A Antibody [ARC2072] (A307778) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3min.

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Figure 3: Immunohistochemistry - Anti-Histone H2A Antibody [ARC2072] (A307778)

Immunohistochemistry analysis of paraffin-embedded rat lung using Anti-Histone H2A Antibody [ARC2072] (A307778) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-Histone H2A Antibody [ARC2072] (A307778)

Immunohistochemistry analysis of paraffin-embedded human brain tissue using Anti-Histone H2A Antibody [ARC2072] (A307778) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.