Anti-Huntingtin Antibody [ARC0431] (A307062)

Name: Anti-Huntingtin Antibody [ARC0431]
Brand: Antibodies.com
SKU: A307062

Enlarge Image

This product is discontinued

Anti-Huntingtin Antibody [ARC0431] (A307062) has been discontinued and is no longer available.

View all Anti-Huntingtin Antibodies.

Specifications

Name

Anti-Huntingtin Antibody [ARC0431]

Description

Rabbit monoclonal [ARC0431] antibody to Huntingtin.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Huntingtin (P42858).

Sequence

MATLEKLMKAFESLKSFQQQQQQQQQQQQQQQQQQQQQPPPPPPPPPPPQLPQPPPQAQPLLPQPQPPPPPPPPPPGPAVAEEPLHRPKKELSATKKDRV

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0431

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

350 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

AI256365, C430023I11Rik, HD, HD protein, HDH, HD_HUMAN, HTT, HUNTINGTON CHOREA, Huntington disease protein, Huntington's disease protein homolog, IT 15, IT15, OTTMUSP00000026909, ZHD

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (6)

Enlarge Image

Figure 1: Western Blot - Anti-Huntingtin Antibody [ARC0431] (A307062)

Western blot analysis of extracts of various cell lines, using Anti-Huntingtin Antibody [ARC0431] (A307062) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

Enlarge Image

Figure 2: Western Blot - Anti-Huntingtin Antibody [ARC0431] (A307062)

Enlarge Image

Figure 3: Immunohistochemistry - Anti-Huntingtin Antibody [ARC0431] (A307062)

Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-Huntingtin Antibody [ARC0431] (A307062) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Enlarge Image

Figure 4: Immunohistochemistry - Anti-Huntingtin Antibody [ARC0431] (A307062)

Immunohistochemistry analysis of paraffin-embedded mouse spinal cord using Anti-Huntingtin Antibody [ARC0431] (A307062) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.