Anti-Nanog Antibody (A9165)

Name: Anti-Nanog Antibody
Brand: Antibodies.com
SKU: A9165
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to Nanog for WB and IHC.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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iPSCs Marker

Specifications

Name

Anti-Nanog Antibody

Description

Rabbit polyclonal antibody to Nanog.

Applications

WB, IHC

Dilutions

WB: 1:1,000-1:2,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-105 of human Nanog (NP_079141.2).

Sequence

MSVDPACPQSLPCFEASDCKESSPMPVICGPEENYPSLQMSSAEMPHTETVSPLPSSMDLLIQDSPDSSTSPKGKQPTSAEKSVAKKEDKVPVKKQKTRTVFSST

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

48 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Embryonic stem cell specific homeobox protein (Nanog), ENK, FLJ12581, hNanog, Homeobox protein NANOG, Homeobox transcription factor Nanog, homeobox transcription factor Nanog-delta 48, Nanog homeobox, NANOG_HUMAN

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (2)

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Figure 1: Western Blot - Anti-Nanog Antibody (A9165)

Western blot analysis of extracts of NIH/3T3 cells, using Anti-Nanog Antibody (A9165) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 45s.

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Figure 2: Immunohistochemistry - Anti-Nanog Antibody (A9165)

Immunohistochemistry analysis of paraffin-embedded human testicular seminoma using Anti-Nanog Antibody (A9165) at a dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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