Anti-NCAM1 Antibody [ARC60182] (A309700)

Name: Anti-NCAM1 Antibody [ARC60182]
Brand: Antibodies.com
SKU: A309700

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This product is discontinued

Anti-NCAM1 Antibody [ARC60182] (A309700) has been discontinued and is no longer available.

View all Anti-NCAM1 Antibodies.

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Anti-CD56 / NCAM Antibody

Rabbit polyclonal antibody to CD56 / NCAM for ELISA, IHC, IP and WB.

Specifications

Name

Anti-NCAM1 Antibody [ARC60182]

Description

Rabbit monoclonal [ARC60182] antibody to NCAM1.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, IF: 1:50-1:200

Reactivity

Mouse, Rat

Immunogen

Recombinant protein of human NCAM /CD56.

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC60182

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

120 - 220 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

antigen MSK39 identified by monoclonal, antigen recognized by monoclonal, CD56, cell adhesion molecule, neural, 1, MSK 39, MSK39, N-CAM-1, NCAM, NCAM 1, NCAM C, NCAM-1, NCAM1_HUMAN, NCAMC, Neural cell adhesion molecule 1, Neural cell adhesion molecule NCAM, OTTHUMP00000235666

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-NCAM1 Antibody [ARC60182] (A309700)

Western blot analysis of various lysates, using Anti-NCAM1 Antibody [ARC60182] (A309700) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 2: Immunohistochemistry - Anti-NCAM1 Antibody [ARC60182] (A309700)

Immunohistochemistry analysis of paraffin-embedded mouse brain using Anti-NCAM1 Antibody [ARC60182] (A309700) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-NCAM1 Antibody [ARC60182] (A309700)

Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-NCAM1 Antibody [ARC60182] (A309700) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-NCAM1 Antibody [ARC60182] (A309700)

Immunohistochemistry analysis of paraffin-embedded rat colon using Anti-NCAM1 Antibody [ARC60182] (A309700) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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