Anti-NF-M Antibody (A85323)

Name: Anti-NF-M Antibody
Brand: Antibodies.com
SKU: A85323
Price: 185.0 USD
Availability: InStock

Enlarge Image

$185

Rabbit polyclonal antibody to NF-M for WB, ICC/IF and IHC.

100% Guarantee

Price Match Guarantee

Delivery

Freight Charges

$40 to United States

Lead Time

4-6 Business Days

Contact Us

Telephone

+1 (314) 370-6046

orders@antibodies.com

Guarantees

100% Quality Guarantee

Price Match Guarantee

Specifications

Name

Anti-NF-M Antibody

Description

Rabbit polyclonal antibody to NF-M.

Applications

WB, ICC/IF, IHC

Dilutions

WB: 1:1,000-1:5,000, ICC/IF: 1:1,000-1:2,500, IHC: 1:1,000-1:2,500

Reactivity

Human, Horse, Bovine, Porcine, Chicken, Rat, Mouse

Immunogen

Recombinant fusion protein corresponding to the extreme C-terminal segment, amino acids 549-845, of rat NF-M.

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Molecular Weight

145-160 kDa

Purity

Whole antiserum.

Product Form

Liquid

Formulation

Supplied as an aliquot of serum with 5mM Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

150kDa medium, 160 kDa neurofilament protein, 2 kDa neurofilament protein, 200 kDa neurofilament protein, 68 kDa neurofilament protein, AI847934, CMT1F, CMT2CC, CMT2E, FLJ53642, KIAA0845, light molecular weight neurofilament protein, Micro glutamic acid-rich protein, mKIAA0845, Nef3, NEFH, NEFL, NEFM, Neurofilament, Neurofilament 3, Neurofilament heavy polypeptide, Neurofilament heavy polypeptide 200kDa, Neurofilament light polypeptide, Neurofilament light polypeptide 68kDa, Neurofilament medium polypeptide, Neurofilament medium polypeptide 150kDa, Neurofilament protein heavy polypeptide, Neurofilament protein light chain, Neurofilament protein light polypeptide, Neurofilament protein medium polypeptide, neurofilament subunit NF-L, Neurofilament triplet H protein, Neurofilament triplet L protein, Neurofilament triplet M protein, neurofilament-3 (150 kD medium), NF-H, NF-L, NF160, NF165, NF200, NF68, NFH, NFL, NFM, NFM_HUMAN, PPP1R110, protein phosphatase 1, regulatory subunit 110

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

Enlarge Image

Figure 1: Immunofluorescence - Anti-NF-M Antibody (A85323)

Immunofluorescent analysis of rat cerebellum section stained with Anti-NF-M Antibody (1:2,000 | red) and Anti-GAP43 Antibody (A85393 | 1:2,000 | green). Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45µM, and free-floating sections were stained with the above antibodies. The Anti-NF-M Antibody strongly labels neuronal processes throughout the cerebellum, while the Anti-GAP43 Antibody stains predominantly synaptic regios in the molecular layer.

Enlarge Image

Figure 2: Immunofluorescence - Anti-NF-M Antibody (A85323)

Mixed neuron/glia cultures stained with Anti-NF-M Antibody (red) and Anti-Fox1 Antibody (A85396 | green). The Anti-NF-M Antibody stains axonal, dendritic and perikaryal profiles of neurons cleanly and specifically. The Anti-Fox1 Antibody binds to the nuclei of neurons only. DNA is shown in blue with DAPI.

Enlarge Image

Figure 3: Western Blot - Anti-NF-M Antibody (A85323)

Western blot analysis of neuronal tissue lysates using Anti-NF-M Antibody (1:2,000 | green): [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] pig brain, [7] pig spinal cord. Strong bands at 145kDa correspond to rodent NF-M molecules, while the NF-M of pig and other larger mammals including humans run at about 160kDa.

Enlarge Image

Figure 4: Western Blot - Anti-NF-M Antibody (A85323)

Blot of rat spinal cord stained with Anti-NF-M Antibody (1: 1,000). A prominent band running with an apparent SDS-PAGE molecular weight of ~145 kDa corresponds to rodent NF-M.

Publishing research using Anti-NF-M Antibody (A85323)? Please let us know so that we can list the citation on this page.