Anti-p53 Antibody (A309435)

Name: Anti-p53 Antibody
Brand: Antibodies.com
SKU: A309435
Price: 470.0 USD
Availability: InStock

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$470

Rabbit polyclonal antibody to p53 for WB, IHC, ICC/IF, IP and ChIP.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Tags:

Knockout Validated Antibody ER Marker Mitochondrial Marker

Specifications

Name

Anti-p53 Antibody

Description

Rabbit polyclonal antibody to p53.

Applications

WB, IHC, ICC/IF, IP, ChIP

Dilutions

WB: 1:500-1:1,000, IHC: 1:100-1:500, ICC/IF: 1:50-1:200, IP: 1:1,000-1:5,000, ChIP: 1:1,000-1:5,000

Reactivity

Human

Immunogen

Recombinant protein of human p53.

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

53 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Antigen NY-CO-13, BCC7, Cellular tumor antigen p53, FLJ92943, LFS1, Mutant tumor protein 53, p53 tumor suppressor, P53_HUMAN, Phosphoprotein p53, Tp53, Transformation related protein 53, TRP53, Tumor protein 53, Tumor protein p53, Tumor suppressor p53

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (7)

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Figure 1: Western Blot - Anti-p53 Antibody (A309435)

Western blot analysis of extracts of 293T cells, using Anti-p53 Antibody (A309435) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 2: Western Blot - Anti-p53 Antibody (A309435)

Western blot analysis of extracts from wild type (WT) and p53 knockout (KO) 293T cells, using Anti-p53 Antibody (A309435) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 3: Immunohistochemistry - Anti-p53 Antibody (A309435)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-p53 Antibody (A309435) at a dilution of 1:300 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunofluorescence - Anti-p53 Antibody (A309435)

Confocal immunofluorescence analysis of Hela cells using Anti-p53 Antibody (A309435) at a dilution of 1:200. DAPI was used to stain the cell nuclei (blue).