Anti-p53 (phospho Ser33) Antibody (A90465)

Name: Anti-p53 (phospho Ser33) Antibody
Brand: Antibodies.com
SKU: A90465
Price: 435.0 USD
Availability: InStock

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$435

Rabbit polyclonal antibody to p53 (phospho Ser33) for WB, IHC and IP.

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$40 to United States

Lead Time

5-8 Business Days

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Tags:

ER Marker Mitochondrial Marker

Specifications

Name

Anti-p53 (phospho Ser33) Antibody

Description

Rabbit polyclonal antibody to p53 (phospho Ser33).

Applications

WB, IHC, IP

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:100, IP: 1:50-1:100

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic phosphorylated peptide around S33 of human p53 (NP_000537.3).

Sequence

VLSPL

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

55 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Antigen NY-CO-13, BCC7, Cellular tumor antigen p53, FLJ92943, LFS1, Mutant tumor protein 53, p53 tumor suppressor, P53_HUMAN, Phosphoprotein p53, Tp53, Transformation related protein 53, TRP53, Tumor protein 53, Tumor protein p53, Tumor suppressor p53

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-p53 (phospho Ser33) Antibody (A90465)

Western blot analysis of extracts of HT-29 cells, using Anti-p53 (phospho Ser33) Antibody (A90465) at 1:2,000 dilution or p53 antibody (A3185). HT-29 cells were treated by Nocodazole (100ng/ml) for 16 hours. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 2: Immunohistochemistry - Anti-p53 (phospho Ser33) Antibody (A90465)

Immunohistochemistry analysis of paraffin-embedded rat liver using Anti-p53 (phospho Ser33) Antibody (A90465) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-p53 (phospho Ser33) Antibody (A90465)

Immunohistochemistry analysis of paraffin-embedded human colon tissue using Anti-p53 (phospho Ser33) Antibody (A90465) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-p53 (phospho Ser33) Antibody (A90465)

Immunohistochemistry analysis of paraffin-embedded mouse liver using Anti-p53 (phospho Ser33) Antibody (A90465) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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A98895

Anti-p53 (phospho Ser33) Antibody (A90465)

Rabbit polyclonal antibody to p53 (phospho Ser33) for WB, IHC and IP.

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