Anti-PAK1 (phospho Thr212) Antibody (A94422)

Western blot analysis of lysates from 293 cells treated with etoposide 25uM 1h using Anti-PAK1 (phospho Thr212) Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Anti-PAK1 (phospho Thr212) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.

Western blot analysis of 293 cells using Anti-PAK1 (phospho Thr212) Antibody.

Immunostaining of frontal cortical sections from E14.5 transgenic embryos expressing tdTomato in MGE-derived cells (Nkx2.1-Cre; Ai9 mouse strain) with anti-activated PAK3-P-Ser154 antibodies. PAK3-P-Ser154 immunoreactivity strongly labels tangentially oriented structures (F1, F2) within the intermediate zone (IZ) and subventricular zone (SVZ), where tdTomato-positive MGE-derived cells undergo tangential migration. Higher-magnification views of cortical progenitors located adjacent to the ventricle (G1–G3) show that PAK3-P-Ser154 antibodies label both the gamma-tubulin-positive centrioles (G2, G3) and the cell cortex (G1, G3) in a subset of progenitor cells. Abbreviations: cx, cortex; hi, hippocampus; th, thalamus; VZ, ventricular zone; SVZ, subventricular zone; IZ, intermediate zone; SP, subplate; CP, cortical plate; MZ, marginal zone. Scale bars: A1, 200 µm; B1, C1, 500 µm; D, 200 µm; E1, 100 µm; F1, 100 µm; G3, 10 µm.