Anti-PAX3 Antibody (A13587)

Name: Anti-PAX3 Antibody
Brand: Antibodies.com
SKU: A13587
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to PAX3 for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

orders@antibodies.com

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Specifications

Name

Anti-PAX3 Antibody

Description

Rabbit polyclonal antibody to PAX3.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 341-479 of human PAX3 (NP_852122.1).

Sequence

QSTIPSNPDSSSAYCLPSTRHGFSSYTDSFVPPSGPSNPMNPTIGNGLSPQVMGLLTNHGGVPHQPQTDYALSPLTGGLEPTTTVSASCSQRLDHMKSLDSLPTSQSYCPPTYSTTGYSMDPVTGYQYGQYGQSKPWTF

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

60 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

CDHS, HUP 2, HUP2, MGC120381, MGC120382, MGC120383, MGC120384, MGC134778, Paired box 3, Paired box gene 3, Paired box homeotic gene 3, Paired box protein Pax 3, Paired box protein Pax-3, Paired box protein Pax3, Paired domain gene 3, Paired domain gene HuP2, PAX 3, PAX3/FKHR fusion gene, PAX3_HUMAN, Sp, splotch, Waardenburg syndrome 1, WS 1, WS1, WS3

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-PAX3 Antibody (A13587)

Western blot analysis of extracts of various cell lines, using Anti-PAX3 Antibody (A13587) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Figure 2: Immunohistochemistry - Anti-PAX3 Antibody (A13587)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-PAX3 Antibody (A13587) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.