Anti-PKC alpha Antibody [ARC0197] (A13227)

Name: Anti-PKC alpha Antibody [ARC0197]
Brand: Antibodies.com
SKU: A13227
Price: 540.0 USD
Availability: InStock

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$540

Rabbit monoclonal [ARC0197] antibody to PKC alpha for WB, IHC and IP.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Specifications

Name

Anti-PKC alpha Antibody [ARC0197]

Description

Rabbit monoclonal [ARC0197] antibody to PKC alpha.

Applications

WB, IHC, IP

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, IP: 1:500-1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 573-672 of human PKC alpha (P17252).

Sequence

MTKHPAKRLGCGPEGERDVREHAFFRRIDWEKLENREIQPPFKPKVCGKGAENFDKFFTRGQPVLTPPDQLVIANIDQSDFEGFSYVNPQFVHPILQSAV

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0197

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

76 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

AAG6, Aging associated gene 6, aPKC, KPCA_HUMAN, PKC-A, PKC-alpha, PKCA, PRKACA, PRKCA, Protein Kinase C alpha, Protein kinase C alpha type

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-PKC alpha Antibody [ARC0197] (A13227)

Western blot analysis of extracts of various cell lines, using Anti-PKC alpha Antibody [ARC0197] (A13227) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 2: Immunohistochemistry - Anti-PKC alpha Antibody [ARC0197] (A13227)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-PKC alpha Antibody [ARC0197] (A13227) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 3: Western Blot - Anti-PKC alpha Antibody [ARC0197] (A13227)

Immunoprecipitation analysis of 300µg extracts of HeLa cells using 3µg of Anti-PKC alpha Antibody [ARC0197] (A13227). This Western blot was performed on the immunoprecipitate using Anti-PKC alpha Antibody [ARC0197] (A13227) at a dilution of 1:1000.

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