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Anti-PKC alpha (phospho Thr638) Antibody [ARC1591] (A309082)

Western Blot - Anti-PKC alpha (phospho Thr638) Antibody [ARC1591] (A309082) - Antibodies.com
$540
Product Datasheet

Rabbit monoclonal [ARC1591] antibody to PKC alpha (phospho Thr638) for WB.

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$40 to United States
Lead Time
5-8 Business Days

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Name
Anti-PKC alpha (phospho Thr638) Antibody [ARC1591]
Description
Rabbit monoclonal [ARC1591] antibody to PKC alpha (phospho Thr638).
Applications
WB
Dilutions
WB: 1:500-1:2,000
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic phosphorylated peptide around T638 of human PKC alpha (P17252).
Sequence
VLTPP
Host
Rabbit
Clonality
Monoclonal
Clone ID
ARC1591
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
80 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
AAG6, Aging associated gene 6, aPKC, KPCA_HUMAN, PKC-A, PKC-alpha, PKCA, PRKACA, PRKCA, Protein Kinase C alpha, Protein kinase C alpha type
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-PKC alpha (phospho Thr638) Antibody [ARC1591] (A309082) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-PKC alpha (phospho Thr638) Antibody [ARC1591] (A309082)
Western blot analysis of extracts of various cell lines, using Anti-PKC alpha (phospho Thr638) Antibody [ARC1591] (A309082) at 1:1,000 dilution. C2C12 cells were treated by PMA/TPA (200 nM) at 37°C for 30 minutes after serum-starvation overnight. C6 cells were treated by CIP(20uL/400ul) at 37°C for 1 hour. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 10s.
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