Anti-PTEN Antibody [ARC2643] (A307897)

Name: Anti-PTEN Antibody [ARC2643]
Brand: Antibodies.com
SKU: A307897
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC2643] antibody to PTEN for WB, IHC, ICC/IF and IP.

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Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Tags:

Knockout Validated Antibody Ribosomal Marker

Specifications

Name

Anti-PTEN Antibody [ARC2643]

Description

Rabbit monoclonal [ARC2643] antibody to PTEN.

Applications

WB, IHC, ICC/IF, IP

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200, IP: 1:500-1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-403 of human PTEN (P60484).

Sequence

MTAIIKEIVSRNKRRYQEDGFDLDLTYIYPNIIAMGFPAERLEGVYRNNIDDVVRFLDSKHKNHYKIYNLCAERHYDTAKFNCRVAQYPFEDHNPPQLELIKPFCEDLDQWLSEDDNHVAAIHCKAGKGRTGVMICAYLLHRGKFLKAQEALDFYGEVRTRDKKGVTIPSQRRYVYYYSYLLKNHLDYRPVALLFHKMMFETIPMFSGGTCNPQFVVCQLKVKIYSSNSGPTRREDKFMYFEFPQPLPVCGDIKVEFFHKQNKMLKKDKMFHFWVNTFFIPGPEETSEKVENGSLCDQEIDSICSIERADNDKEYLVLTLTKNDLDKANKDKANRYFSPNFKVKLYFTKTVEEPSNPEASSSTSVTPDVSDNEPDHYRYSDTTDSDPENEPFDEDQHTQITKV

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC2643

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

54 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

10q23del, BZS, DEC, GLM2, MGC11227, MHAM, MMAC1, MMAC1 phosphatase and tensin homolog deleted on chromosome 10, Mutated in multiple advanced cancers 1, Phosphatase and tensin homolog, Phosphatase and tensin like protein, Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN, PTEN1, PTEN_HUMAN, TEP1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (6)

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Figure 1: Western Blot - Anti-PTEN Antibody [ARC2643] (A307897)

Western blot analysis of extracts of various cell lines, using Anti-PTEN Antibody [ARC2643] (A307897) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 2: Western Blot - Anti-PTEN Antibody [ARC2643] (A307897)

Western blot analysis of extracts of C6 cells, using Anti-PTEN Antibody [ARC2643] (A307897) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.

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Figure 3: Western Blot - Anti-PTEN Antibody [ARC2643] (A307897)

Western blot analysis of extracts from wild type (WT) and PTEN knockout (KO) HeLa cells, using Anti-PTEN Antibody [ARC2643] (A307897) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.

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Figure 4: Immunohistochemistry - Anti-PTEN Antibody [ARC2643] (A307897)

Immunohistochemistry analysis of paraffin-embedded human breast cancer tissue using Anti-PTEN Antibody [ARC2643] (A307897) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.