Anti-S6K1 (phospho Thr389) Antibody (A305998)

Name: Anti-S6K1 (phospho Thr389) Antibody
Brand: Antibodies.com
SKU: A305998
Price: 435.0 USD
Availability: InStock

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$435

Rabbit polyclonal antibody to S6K1 (phospho Thr389) for WB and IHC.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Tags:

Ribosomal Marker

Specifications

Name

Anti-S6K1 (phospho Thr389) Antibody

Description

Rabbit polyclonal antibody to S6K1 (phospho Thr389).

Applications

WB, IHC

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic phosphorylated peptide around T389 of human P70S6K1 (NP_001258989.1?).

Sequence

GFTYV

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

70 kDa / 85 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

70 kDa ribosomal protein S6 kinase 1, KS6B1_HUMAN, p70 alpha, P70 beta 1, p70 ribosomal S6 kinase alpha, p70 ribosomal S6 kinase beta 1, P70 S6 Kinase, p70 S6 kinase alpha, p70 S6 kinase, alpha 1, p70 S6 kinase, alpha 2, p70 S6K, p70 S6K-alpha, p70 S6KA, p70(S6K) alpha, p70(S6K)-alpha, p70-alpha, p70-S6K, p70-S6K 1, P70S6K, P70S6K1, p70S6Kb, PS6K, Ribosomal protein S6 kinase 70kDa polypeptide 1, Ribosomal protein S6 kinase beta 1, Ribosomal protein S6 kinase beta-1, Ribosomal protein S6 kinase I, RPS6KB1, S6K, S6K-beta-1, Serine/threonine kinase 14 alpha, Serine/threonine-protein kinase 14A, STK14A

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (6)

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Figure 1: Western Blot - Anti-S6K1 (phospho Thr389) Antibody (A305998)

Western blot analysis of NIH/3T3, using Anti-S6K1 (phospho Thr389) Antibody (A305998) at 1:500 dilution. NIH/3T3 cells were treated by 10% FBS at 37°C for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 2: Western Blot - Anti-S6K1 (phospho Thr389) Antibody (A305998)

Western blot analysis of C6, using Anti-S6K1 (phospho Thr389) Antibody (A305998) at 1:500 dilution. C6 cells were treated by Î»-PP mixed solution (1ul) at 30°C for 30 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 3: Immunohistochemistry - Anti-S6K1 (phospho Thr389) Antibody (A305998)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-S6K1 (phospho Thr389) Antibody (A305998) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-S6K1 (phospho Thr389) Antibody (A305998)

Immunohistochemistry analysis of paraffin-embedded human colon tissue using Anti-S6K1 (phospho Thr389) Antibody (A305998) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.