Anti-S6K1 (phospho Thr421 + Ser424) Antibody [ARC0107] (A10982)

Name: Anti-S6K1 (phospho Thr421 + Ser424) Antibody [ARC0107]
Brand: Antibodies.com
SKU: A10982
Price: 540.0 USD
Availability: InStock

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$540

Rabbit monoclonal [ARC0107] antibody to S6K1 (phospho Thr421 + Ser424) for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Tags:

Ribosomal Marker

Specifications

Name

Anti-S6K1 (phospho Thr421 + Ser424) Antibody [ARC0107]

Description

Rabbit monoclonal [ARC0107] antibody to S6K1 (phospho Thr421 + Ser424).

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide derived from human Phospho-S6K1 (T421 + S424).

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0107

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

70 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

70 kDa ribosomal protein S6 kinase 1, KS6B1_HUMAN, p70 alpha, P70 beta 1, p70 ribosomal S6 kinase alpha, p70 ribosomal S6 kinase beta 1, P70 S6 Kinase, p70 S6 kinase alpha, p70 S6 kinase, alpha 1, p70 S6 kinase, alpha 2, p70 S6K, p70 S6K-alpha, p70 S6KA, p70(S6K) alpha, p70(S6K)-alpha, p70-alpha, p70-S6K, p70-S6K 1, P70S6K, P70S6K1, p70S6Kb, PS6K, Ribosomal protein S6 kinase 70kDa polypeptide 1, Ribosomal protein S6 kinase beta 1, Ribosomal protein S6 kinase beta-1, Ribosomal protein S6 kinase I, RPS6KB1, S6K, S6K-beta-1, Serine/threonine kinase 14 alpha, Serine/threonine-protein kinase 14A, STK14A

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (7)

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Figure 1: Western Blot - Anti-S6K1 (phospho Thr421 + Ser424) Antibody [ARC0107] (A10982)

Western blot analysis of extracts of various cell lines, using Anti-S6K1 (phospho Thr421 + Ser424) Antibody [ARC0107] (A10982) at 1:1,000 dilution. NIH/3T3 and C6 cells were treated by 10% FBS at 37°C for 30 minutes after serum-starvation overnightThe secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60S.

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Figure 2: Immunohistochemistry - Anti-S6K1 (phospho Thr421 + Ser424) Antibody [ARC0107] (A10982)

Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-S6K1 (phospho Thr421 + Ser424) Antibody [ARC0107] (A10982) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-S6K1 (phospho Thr421 + Ser424) Antibody [ARC0107] (A10982)

Immunohistochemistry analysis of paraffin-embedded human oophoroma using Anti-S6K1 (phospho Thr421 + Ser424) Antibody [ARC0107] (A10982) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-S6K1 (phospho Thr421 + Ser424) Antibody [ARC0107] (A10982)

Immunohistochemistry analysis of paraffin-embedded mouse testis using Anti-S6K1 (phospho Thr421 + Ser424) Antibody [ARC0107] (A10982) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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