Rabbit polyclonal antibody to SHC1 (phospho Y349).
Applications
WB, IF
Dilutions
WB: 1:500-1:2000, IF: 1:100-1:200
Reactivity
Human, Mouse
Immunogen
A phospho specific peptide corresponding to residues surrounding Y349 of human SHC1.
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Affinity purification.
Molecular Weight
50kDa
Formulation
Supplied in Phosphate Buffered Saline, pH 7.30, with 0.02% Sodium Azide and 50% Glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
SH2 domain protein C1, SHC-transforming protein 1, SHC-transforming protein 3, SHC-transforming protein A, SHCA, Src homology 2 domain-containing-transforming protein C1
Western Blot - Anti-SHC1 (phospho Y349) Antibody (A12474)
Western blot analysis of extracts from HepG2 cells using Anti-SHC1 (phospho Y349) Antibody (AP0265). Secondary antibody: Goat Anti-Rabbit IgG (H+L) (HRP) (AS014) at 1:10,000 dilution. Lysates / proteins: 25µg per lane. Blocking buffer: 3% BSA.
