Anti-STAT3 Antibody (A13365)

Name: Anti-STAT3 Antibody
Brand: Antibodies.com
SKU: A13365
Price: 335.0 USD
Availability: InStock

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This product is discontinued

Anti-STAT3 Antibody (A13365) has been discontinued and is no longer available.

View all Anti-STAT3 Antibodies.

Specifications

Name

Anti-STAT3 Antibody

Description

Rabbit polyclonal antibody to STAT3.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 600-700 of human STAT3 (NP_003141.2).

Sequence

TKPPGTFLLRFSESSKEGGVTFTWVEKDISGKTQIQSVEPYTKQQLNNMSFAEIIMGYKIMDATNILVSPLVYLYPDIPKEEAFGKYCRPESQEHPEADPG

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

86 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

1110034C02Rik, Acute Phase Response Factor, Acute-phase response factor, ADMIO, APRF, AW109958, DNA binding protein APRF, FLJ20882, HIES, MGC16063, Signal transducer and activator of transcription 3, Signal transducer and activator of transcription 3 (acute phase response factor), STAT 3, STAT3_HUMAN

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (11)

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Figure 1: Western Blot - Anti-STAT3 Antibody (A13365)

Western blot analysis of various lysates, using Anti-STAT3 Antibody (A13365) at 1:2,500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.

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Figure 2: Immunohistochemistry - Anti-STAT3 Antibody (A13365)

Immunohistochemistry analysis of paraffin-embedded mouse brain using Anti-STAT3 Antibody (A13365) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-STAT3 Antibody (A13365)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-STAT3 Antibody (A13365) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-STAT3 Antibody (A13365)

Immunohistochemistry analysis of paraffin-embedded rat heart using Anti-STAT3 Antibody (A13365) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.