Immunofluorescent analysis of rat brain section stained with Anti-Tyrosine Hydroxylase Antibody (1:10,000 | red) and co-stained with Anti-NF-H Antibody (1:1,000 | green). The blue is Hoechst staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45µM, and free-floating sections were stained with the above antibodies. The Anti-Tyrosine Hydroxylase Antibody stains the striatal TH expressing interneurons, while the Anti-NF-H Antibody labels axons from other neuronal cells.
Figure 2: Western Blot - Anti-Tyrosine Hydroxylase Antibody (A104319)
Western blot analysis of different tissue and cell lysates using Anti-Tyrosine Hydroxylase Antibody (A104319), at a dilution of 1:5,000, in green. The lanes contain samples of: [1] Protein standards, in red, [2] rat brain, [3] mouse brain, [4] SH-SY5Y cells, and [5] PC12 cells. The strong band at about 60 kDa corresponds to TH protein expected in brain and PC12 extracts, but not in SH-SY5Y cells.
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