Figure 1: Western Blot - Anti-Tyrosine Hydroxylase Antibody (A83430)
Anti-TH Antibody (A83430) (2µg/ml) staining of Human Cerebral Cortex lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB6739 (0.1µg/ml) staining of PFA-perfused cryosection of Human Hypothalamic supraoptic (SO), paraventricular (Pa) and periventricular (Pe) nuclei. Antigen retrieval with citrate buffer pH 6 at 80C for 30min, HRP-staining with Ni-DAB after Biotin-SP-antigoat amplification.
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