Anti-UBE2C Antibody (A14807)

Name: Anti-UBE2C Antibody
Brand: Antibodies.com
SKU: A14807
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to UBE2C for WB, IHC, ICC/IF and IP.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Specifications

Name

Anti-UBE2C Antibody

Description

Rabbit polyclonal antibody to UBE2C.

Applications

WB, IHC, ICC/IF, IP

Dilutions

WB: 1:100-1:500, IHC: 1:50-1:200, ICC/IF: 1:50-1:200, IP: 1:500-1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-179 of human UBE2C (NP_008950.1).

Sequence

MASQNRDPAATSVAAARKGAEPSGGAARGPVGKRLQQELMTLMMSGDKGISAFPESDNLFKWVGTIHGAAGTVYEDLRYKLSLEFPSGYPYNAPTVKFLTPCYHPNVDTQGNICLDILKEKWSALYDVRTILLSIQSLLGEPNIDSPLNTHAAELWKNPTAFKKYLQETYSKQVTSQEP

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

21 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Cyclin selective ubiquitin carrier protein, dJ447F3.2, Mitotic specific ubiquitin conjugating enzyme, UB E2C, UBCH 10, UbcH10, UBE 2C, UBE2C_HUMAN, Ubiquitin carrier protein C, Ubiquitin carrier protein E2 C, Ubiquitin carrier protein E2C, Ubiquitin conjugating enzyme E2 C, Ubiquitin conjugating enzyme E2C, Ubiquitin protein ligase C, Ubiquitin-conjugating enzyme E2 C, Ubiquitin-protein ligase C

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (6)

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Figure 1: Western Blot - Anti-UBE2C Antibody (A14807)

Western blot analysis of various lysates, using Anti-UBE2C Antibody (A14807) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 90s.

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Figure 2: Immunohistochemistry - Anti-UBE2C Antibody (A14807)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-UBE2C Antibody (A14807) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-UBE2C Antibody (A14807)

Immunohistochemistry analysis of paraffin-embedded human placenta using Anti-UBE2C Antibody (A14807) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunofluorescence - Anti-UBE2C Antibody (A14807)

Immunofluorescence analysis of A-549 cells using Anti-UBE2C Antibody (A14807) at a dilution of 1:50 (40x lens). DAPI was used to stain the cell nuclei (blue).