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Anti-Ubiquitin (phospho Ser65) Antibody (A309718)

Western Blot - Anti-Ubiquitin (phospho Ser65) Antibody (A309718) - Antibodies.com
$435
Product Datasheet

Rabbit polyclonal antibody to Ubiquitin (phospho Ser65) for WB.

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Anti-Ubiquitin Antibody
Rabbit polyclonal antibody to Ubiquitin for WB, ICC/IF and IHC.
Name
Anti-Ubiquitin (phospho Ser65) Antibody
Description
Rabbit polyclonal antibody to Ubiquitin (phospho Ser65).
Applications
WB
Dilutions
WB: 1:100-1:500
Reactivity
Mouse
Immunogen
A synthetic phosphorylated peptide around S65 of human Ubiquitin (NP_002945.1).
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
Epididymis secretory protein Li 50, FLJ25987, HEL S 50, MGC8385, Polyubiquitin B, RPS 27A, RPS27A, UBA 52, UBA 80, UBA52, UBA80, UBB, UBB_HUMAN, UBC, UBCEP 1, UBCEP 2, UBCEP1, UBCEP2, Ubiquitin B, UBIQ_HUMAN
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-Ubiquitin (phospho Ser65) Antibody (A309718) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-Ubiquitin (phospho Ser65) Antibody (A309718)
Western blot analysis of PC-3, using Anti-Ubiquitin (phospho Ser65) Antibody (A309718) at 1:400 dilution. PC-3 cells were treated by Carbonyl cyanide 3-chlorophenylhydrazone(10 uM) (10 µM) at 37°C for 6 hours after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 60s.
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