Figure 1: Western Blot - Anti-AKT1 (phospho Thr450) Antibody (A16395)
Western blot analysis of extracts of 293 and HeLa cells, using Anti-AKT1 (phospho Thr450) Antibody (A16395) at 1:1,000 dilution. 293 cells were treated by Insulin (100nM) for 10 minutes after serum-starvation overnight. HeLa cells were treated by EGF (100ng/ml) for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 1min.
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