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Anti-AKT1 (phospho Thr450) Antibody [ARC1524] (A308903)

Western Blot - Anti-AKT1 (phospho Thr450) Antibody [ARC1524] (A308903) - Antibodies.com
$540
Product Datasheet

Rabbit monoclonal [ARC1524] antibody to AKT1 (phospho Thr450) for WB.

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$40 to United States
Lead Time
5-8 Business Days

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Name
Anti-AKT1 (phospho Thr450) Antibody [ARC1524]
Description
Rabbit monoclonal [ARC1524] antibody to AKT1 (phospho Thr450).
Applications
WB
Dilutions
WB: 1:500-1:2,000
Reactivity
Human, Rat
Immunogen
A synthetic phosphorylated peptide around T450 of human Akt1 (P31749).
Sequence
TITPP
Host
Rabbit
Clonality
Monoclonal
Clone ID
ARC1524
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
60 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
AKT, AKT 1, AKT1_HUMAN, MGC99656, PKB, PKB-ALPHA, PRKBA, Protein kinase B, Protein Kinase B Alpha, Proto-oncogene c-Akt, RAC, RAC Alpha, RAC-alpha serine/threonine-protein kinase, RAC-PK-alpha
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-AKT1 (phospho Thr450) Antibody [ARC1524] (A308903) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-AKT1 (phospho Thr450) Antibody [ARC1524] (A308903)
Western blot analysis of extracts of C6 cells, using Anti-AKT1 (phospho Thr450) Antibody [ARC1524] (A308903) at 1:1,000 dilution. C6 cells were treated by CIP(20uL/400ul) at 37°C for 1 hour or treated by PDGF (50 ng/ml) at 37°C for 30 minutes after serum-starvation overnightThe secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 3min.
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