Anti-alpha Internexin Antibody (A85441)

Immunofluorescent analysis of rat cerebellum section stained with Anti-Alpha-Internexin Antibody (1:2,000 | green) and Anti-GFAP Antibody (A85307 | 1:5,000 | red). Blue is DAPI staining of nuclear DNA. Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 µM, and free-floating sections were stained with the above antibodies. The Anti-Alpha-Internexin Antibody selectively stains axons and dendrites of neuronal cells, in particular Purkinje cells and parallel fibers the axons of granule cells. The Anti-GFAP Antibody labels network of glial cells, such as astrocytes in the granule cell layer and white matter and Bergmann glia in the molecular layer.

Mixed neuron-glial cultures stained with Anti-Alpha-Internexin Antibody (red) and Anti-Peripherin Antibody (A85435 | green). The Anti-Alpha-Internexin Antibody stains numerous axonal and dendritic profiles in these cultures, while Anti-Peripherin Antibody binds to only a subset of neurons.

Western blot analysis of whole tissue lysates using Anti-Alpha-Internexin Antibody (1:10,000 | green): [1] protein standard (red), [2] mouse spinal cord, [3] rat spinal cord, [4] bovine spinal cord. Major bands in the 64-66 kDa range corresponds to a-internexin. The a-internexin protein from different species is known to vary slightly in SDS-PAGE molecular weight.

Western blot of whole rat spinal cord homogenate stained with Anti-Alpha-Internexin Antibody (1:20,000). A prominent band running at ~66 kDa is apparent, as well as smaller lower bands which are apparently degradation products. A minor band at ~150 kDa is also seen, apparently resulting from dimerization of alpha-internexin.