Anti-alpha Internexin Antibody [2E3] (A85448)

Name: Anti-alpha Internexin Antibody [2E3]
Brand: Antibodies.com
SKU: A85448
Price: 115.0 GBP
Availability: InStock

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$190

Mouse monoclonal [2E3] antibody to alpha Internexin for WB, ICC/IF and IHC.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Specifications

Name

Anti-alpha Internexin Antibody [2E3]

Description

Mouse monoclonal [2E3] antibody to alpha Internexin.

Applications

WB, ICC/IF, IHC

Dilutions

WB: 1:10,000, ICC/IF: 1:1,000-5,000, IHC: 1:1,000-5,000

Reactivity

Human, Rat, Mouse, Bovine

Immunogen

Recombinant full-length rat alpha Internexin, expressed in and purified from E. coli.

Host

Mouse

Clonality

Monoclonal

Clone ID

2E3

Isotype

IgG1

Conjugate

Unconjugated

Purification

Immunogen affinity purification.

Concentration

1 mg/ml

Molecular Weight

64-66 kDa (by SDS-PAGE)

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline with 50% Glycerol and 5mM Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

66 kDa neurofilament protein, Alpha-internexin, Alpha-Inx, INA, NEF5, Neurofilament 5, Neurofilament-66, NF-66

Isotype Controls

Suitable Secondaries

See all Anti-Mouse IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (6)

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Immunohistochemistry - Anti-alpha Internexin Antibody [2E3] (A85448)

Immunohistochemistry of a section of rat facial nucleus 7 days following axotomy using Anti-Alpha-Internexin Antibody. These neurons are capable of regenerating their axons and also, concomitant with regeneration, strongly upregulate a-internexin in their perikarya. Other central neurons which are not able to regenerate their axons do not upregulate this protein after axotomy and untreated facial neurons normally show only very low levels of a-internexin. Both findings suggest that a-internexin has a role in axonal regeneration.

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Immunohistochemistry - Anti-alpha Internexin Antibody [2E3] (A85448)

Rat facial nucleus before (left) and after (right) facial nerve lesion. Note the profound upregulation of a-internexin in the facial nucleus motor neurons.

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Western Blot - Anti-alpha Internexin Antibody [2E3] (A85448)

Western blot analysis of different tissue lysates using Anti-alpha Internexin Antibody [2E3] (A85448), at a dilution of 1:10,000, in red. The lanes contain samples of: [Lane 1] Protein standards, in red, [Lane 2] rat brain, [Lane 3] rat spinal cord, [Lane 4] mouse brain, [Lane 5] mouse spinal cord, and [Lane 6] cow spinal cord lysates. The Anti-alpha Internexin Antibody [2E3] (A85448) reveals the alpha Internexin protein with an apparent molecular weight of 64-66 kDa, with some variability between species.

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Immunohistochemistry - Anti-alpha Internexin Antibody [2E3] (A85448)

Immunohistochemistry analysis of a formalin fixed paraffin embedded human cerebellum section with Anti-alpha Internexin Antibody [2E3] (A85448) at a dilution of 1:1,000 detected in DAB (brown) using the Vector Labs ImmPRESS method and reagents with citra buffer retrieval. Counterstained with Hematoxylin (blue). The 2E3 a-Internexin antibody strongly labels neuronal processes in both rat and human brain. Note: this antibody performs well in testing with both 4% PFA and standard NBF fixed tissues.