Anti-alpha Internexin Antibody [1D2] (A85447)

Mixed cultures of rat CNS cells stained with Anti-a-Internexin Antibody (red) and Anti-MAP2 Antibody (A85363 | green). The a-internexin is localized primarily in neuronal axons in these cultures, while the perikarya and dendrites of neurons stain strongly for MAP2.

Left three lanes show Coomassie brilliant blue stained extracts of rat brain stem, cat cerebral cortex and human cerebral cortex (R, C and H respectively). Right three lanes are corresponding blots reacted with Anti-a-Internexin Antibody. Anti-a-Internexin Antibody stains a band of about 66kDa in all three preparations.

Immunofluorescent analysis of rat cerebellum section stained with Anti-alpha Internexin Antibody [1D2] (A85447), at a dilution of 1:5,000, in green, and co-stained with Anti-Calretinin Antibody (A104312), 1:2,000 in red. Following transcardial perfusion of the rat with 4% paraformaldehyde, the brain was post-fixed for 24 hours, cut to 45 µm, and free-floating sections were stained with the above antibodies. The Anti-alpha Internexin Antibody [1D2] (A85447) selectively stains neuronal processes, in particular parallel fibers, the axons of granule cells. The Anti-Calretinin Antibody (A104312) stains interneurons predominantly in the molecular layer of the cerebellum.

Western blot analysis of different tissue lysates using Anti-alpha Internexin Antibody [1D2] (A85447), at a dilution of 1:10,000, in green. The lanes contain samples of: [Lane 1] Protein standards, [Lane 2] rat brain, [Lane 3] rat spinal cord, [Lane 4] mouse brain, [Lane 5] mouse spinal cord, [Lane 6] pig spinal cord, and [Lane 7] cow spinal cord. The Anti-alpha Internexin Antibody [1D2] (A85447) reveals the alpha Internexin protein with an apparent molecular weight of 64-66 kDa, with slight variability between species.