Rachel Stewart is a former cell and developmental biologist-turned science writer. She completed her PhD in the Cell Biology Department at Yale University, where she examined how crosstalk between the cell nucleus, the cytoskeleton, and cellular adhesions influences global cell and tissue behaviors. She subsequently worked as a postdoctoral researcher in Japan at the RIKEN Center for Biosystems Dynamics Research, studying how interactions between intercellular adhesions and the actin cytoskeleton regulate aspects of early embryo development in Drosophila. She now lives in Edinburgh and works as a senior content marketing manager at Qiagen.
Rachel enjoys communicating science for a broad range of audiences, including, children, doctors, scientists, government leaders and the general public, as well as exploring the intersection between science and art.
Western blotting uses antibodies to selectively label proteins of interest from lysed cell or tissue samples to measure size and abundance...
A detailed overview of western blotting protocols including: sample preparation, gel electrophoresis, protein transfer, and immunodetection, protocols...
A detailed overview of western blotting troubleshooting, including issues with no bands, disrupted membrane appearance, uneven loading controls and unexpected results...
Immunohistochemistry (IHC) is a technique that uses antibody-epitope interactions to selectively label and visualize proteins in tissue samples...
A detailed overview of Immunohistochemistry protocols, including sample preparation, fixation, antigen retrieval, and staining...
A detailed overview of Immunohistochemistry troubleshooting, including solutions for weak signal, high background and disrupted tissue morphology...
A detailed guide to Immunohistochemistry controls, including positive and negative controls, and controls for specificity and endogenous background...
Learn about the cellular and molecular biology of the nucleus and the most popular nuclear markers...
