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Anti-Bcl-2 Antibody (A305144)

Immunohistochemistry - Anti-Bcl-2 Antibody (A305144) - Antibodies.com Western Blot - Anti-Bcl-2 Antibody (A305144) - Antibodies.com Immunohistochemistry - Anti-Bcl-2 Antibody (A305144) - Antibodies.com
$500
Product Datasheet

Rabbit polyclonal antibody to Bcl-2 for WB and IHC.

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A86278
16 Citations
Anti-Bcl-2 Antibody [Bcl-2/100]
Mouse monoclonal [Bcl-2/100] antibody to Bcl-2 for Flow Cytometry, IP, WB, IHC-P, IHC-Fr and ICC.
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A86278
16 Citations
Anti-Bcl-2 Antibody [Bcl-2/100]
Mouse monoclonal [Bcl-2/100] antibody to Bcl-2 for Flow Cytometry, IP, WB, IHC-P, IHC-Fr and ICC.
Name
Anti-Bcl-2 Antibody
Description
Rabbit polyclonal antibody to Bcl-2.
Applications
WB, IHC
Dilutions
WB: 1:1,000, IHC: 1:50
Reactivity
Human, Rat
Immunogen
Synthetic peptide from the mid-protein of Human Bcl-2.
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Peptide affinity purification.
Concentration
1 mg/ml
Molecular Weight
~26 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 50% Glycerol and 0.09% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
Apoptosis regulator Bcl 2, Apoptosis regulator Bcl-2, Apoptosis regulator Bcl2, AW986256, B cell CLL/lymphoma 2, B cell leukemia/lymphoma 2, Bcl2, BCL2_HUMAN, C430015F12Rik, D630044D05Rik, D830018M01Rik, Leukemia/lymphoma, B-cell, 2, Oncogene B-cell leukemia 2, PPP1R50, Protein phosphatase 1, regulatory subunit 50
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Immunohistochemistry - Anti-Bcl-2 Antibody (A305144) - Antibodies.comEnlarge Image
Figure 1: Immunohistochemistry - Anti-Bcl-2 Antibody (A305144)
Immunohistochemistry analysis of human colon, fixed in formalin and paraffin-embedded. The Primary Antibody used was Anti-Bcl-2 Antibody (A305144) at 1:50 for 30 minutes at room temperature. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection.
Western Blot - Anti-Bcl-2 Antibody (A305144) - Antibodies.comEnlarge Image
Figure 2: Western Blot - Anti-Bcl-2 Antibody (A305144)
Western blot analysis of human Embryonic kidney epithelial cell line (HEK293T) lysate showing detection of ~26.3 kDa Bcl-2 protein using Anti-Bcl-2 Antibody (A305144) at 1:1,000 for 2 hours at room temperature. Lane 1: Molecular Weight Ladder (MW). Lane 2: 293Trap cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. The secondary antibody used was Goat Anti-Rabbit IgG: HRP at 1:1,000 for 60 minutes at room temperature. Color Development: ECL solution for 6 min in room temperature. Predicted/Observed Size: ~26.3 kDa.
Immunohistochemistry - Anti-Bcl-2 Antibody (A305144) - Antibodies.comEnlarge Image
Figure 3: Immunohistochemistry - Anti-Bcl-2 Antibody (A305144)
Immunohistochemistry analysis of human colon, fixed in formalin and paraffin-embedded. The Primary Antibody used was Anti-Bcl-2 Antibody (A305144) at 1:50 for 30 minutes at room temperature. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection.
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