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Anti-Bcl-2 Antibody (A88945)

Western Blot - Anti-Bcl-2 Antibody (A88945) - Antibodies.com Western Blot - Anti-Bcl-2 Antibody (A88945) - Antibodies.com Immunohistochemistry - Anti-Bcl-2 Antibody (A88945) - Antibodies.com
This product is discontinued

Anti-Bcl-2 Antibody (A88945) has been discontinued and is no longer available.

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A86278
16 Citations
Anti-Bcl-2 Antibody [Bcl-2/100]
Mouse monoclonal [Bcl-2/100] antibody to Bcl-2 for Flow Cytometry, IP, WB, IHC-P, IHC-Fr and ICC.
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A86278
16 Citations
Anti-Bcl-2 Antibody [Bcl-2/100]
Mouse monoclonal [Bcl-2/100] antibody to Bcl-2 for Flow Cytometry, IP, WB, IHC-P, IHC-Fr and ICC.
Name
Anti-Bcl-2 Antibody
Description
Rabbit polyclonal antibody to Bcl-2.
Applications
WB, IHC, ICC/IF
Dilutions
WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Bcl-2 (NP_000624.2).
Sequence
MAHAGRTGYDNREIVMKYIHYKLSQRGYEWDAGDVGAAPPGAAPAPGIFSSQPGHTPHPAASRDPVARTSPLQTPAAPGAAAGPALSPVPPVVHLTLRQA
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
24 kDa / 26 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
Apoptosis regulator Bcl 2, Apoptosis regulator Bcl-2, Apoptosis regulator Bcl2, AW986256, B cell CLL/lymphoma 2, B cell leukemia/lymphoma 2, Bcl2, BCL2_HUMAN, C430015F12Rik, D630044D05Rik, D830018M01Rik, Leukemia/lymphoma, B-cell, 2, Oncogene B-cell leukemia 2, PPP1R50, Protein phosphatase 1, regulatory subunit 50
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-Bcl-2 Antibody (A88945) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-Bcl-2 Antibody (A88945)
Western blot analysis of extracts of various cells, using Anti-Bcl-2 Antibody (A88945) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.
Western Blot - Anti-Bcl-2 Antibody (A88945) - Antibodies.comEnlarge Image
Figure 2: Western Blot - Anti-Bcl-2 Antibody (A88945)
Western blot analysis of extracts of HCT116 cells, using Anti-Bcl-2 Antibody (A88945) at 1:1,000 dilution. HCT116 cells treated with different concentrations of IL-8. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry - Anti-Bcl-2 Antibody (A88945) - Antibodies.comEnlarge Image
Figure 3: Immunohistochemistry - Anti-Bcl-2 Antibody (A88945)
Immunohistochemistry analysis of paraffin-embedded human stomach using Anti-Bcl-2 Antibody (A88945) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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