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Anti-Akt1 S1 (phospho Thr246) Antibody (A93543)

Western Blot - Anti-Akt1 S1 (phospho Thr246) Antibody (A0453) - Antibodies.com
This product is discontinued

Anti-Akt1 S1 (phospho Thr246) Antibody (A93543) has been discontinued and is no longer available.

View all PRAS40 Antibodies.

  • Specifications

Name
Anti-Akt1 S1 (phospho Thr246) Antibody
Description
Rabbit polyclonal antibody to Akt1 S1 (phospho Thr246).
Specificity
This antibody detects endogenous levels of Akt1 S1 only when phosphorylated at Thr246.
Applications
WB, ELISA
Dilutions
WB: 1:500~1:1000, IHC: 1:50~1:100, ELISA: 1:10000
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide derived from human Akt1 S1 around the phosphorylation site of Thr246 (amino acids 207-256).
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.
Concentration
1mg/ml
Molecular Weight
27kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
40 kDa proline-rich AKT substrate, AKT1S1, Proline-rich AKT1 substrate 1
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (1)

Western Blot - Anti-Akt1 S1 (phospho Thr246) Antibody (A0453) - Antibodies.comEnlarge Image

Western Blot - Anti-Akt1 S1 (phospho Thr246) Antibody (A93543)

Western blot analysis of lysates from HepG2 cells treated with PDGF 50ng/ml 30' using Anti-Akt1 S1 (phospho Thr246) Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.

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Proteins predicted to interact with PRAS40

Predicted protein interactions based upon String database. Revelancy score correlates with probability of interaction.
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.8% Relevancy Score
99.7% Relevancy Score
99.5% Relevancy Score
99.4% Relevancy Score
99.3% Relevancy Score