Western blot analysis of extracts of various cell lines, using Anti-PRAS40 Antibody (A11941) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded human mammary cancer using Anti-PRAS40 Antibody (A11941) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human stomach using Anti-PRAS40 Antibody (A11941) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunofluorescence analysis of NIH-3T3 cells using Anti-PRAS40 Antibody (A11941) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Publishing research using Anti-PRAS40 Antibody (A11941)? Please let us know so that we can list the citation on this page.
Alternative products to Anti-PRAS40 Antibody (A11941)
![Western Blot - Anti-PRAS40 (Phospho T246) Antibody [ARC66323] (A329772) - Antibodies.com](https://cdn.antibodies.com/image/catalog/329/A329772_1.jpg?profile=product_alternative)
![Western Blot - Anti-PRAS40 Antibody [ARC1965] (A308817) - Antibodies.com](https://cdn.antibodies.com/image/catalog/308/A308817_1.jpg?profile=product_alternative)
