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Anti-Akt2 (phospho Ser474) Antibody (A94466)

Western Blot - Anti-Akt2 (phospho Ser474) Antibody (A7006) - Antibodies.com Immunohistochemistry - Anti-Akt2 (phospho Ser474) Antibody (A7006) - Antibodies.com Western Blot - Anti-Akt2 (phospho Ser474) Antibody (A7006) - Antibodies.com Western Blot - Anti-Akt2 (phospho Ser474) Antibody (A7006) - Antibodies.com
This product is discontinued

Anti-Akt2 (phospho Ser474) Antibody (A94466) has been discontinued and is no longer available.

View all AKT2 Antibodies.

  • Specifications

Name
Anti-Akt2 (phospho Ser474) Antibody
Description
Rabbit polyclonal antibody to Akt2 (phospho Ser474).
Specificity
This antibody detects endogenous levels of Akt2 only when phosphorylated at Ser474.
Applications
WB, IHC, ELISA
Dilutions
WB: 1:500~1:1000, IHC: 1:50~1:100, ELISA: 1:20000
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide derived from human Akt2 around the phosphorylation site of Ser474 (amino acids 432-481).
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.
Molecular Weight
55kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
PKB beta, Protein kinase Akt-2, Protein kinase B beta, RAC protein kinase beta, RAC-beta serine/threonine-protein kinase, RAC-PK-beta
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (4)

Western Blot - Anti-Akt2 (phospho Ser474) Antibody (A7006) - Antibodies.comEnlarge Image

Western Blot - Anti-Akt2 (phospho Ser474) Antibody (A94466)

Western blot analysis of lysates from 293 cells treated with EGF 200ng/ml 30' using Anti-Akt2 (phospho Ser474) Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.
Immunohistochemistry - Anti-Akt2 (phospho Ser474) Antibody (A7006) - Antibodies.comEnlarge Image

Immunohistochemistry - Anti-Akt2 (phospho Ser474) Antibody (A94466)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Anti-Akt2 (phospho Ser474) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.
Western Blot - Anti-Akt2 (phospho Ser474) Antibody (A7006) - Antibodies.comEnlarge Image

Western Blot - Anti-Akt2 (phospho Ser474) Antibody (A94466)

Western blot analysis of various cells using Anti-Akt2 (phospho Ser474) Antibody.
Western Blot - Anti-Akt2 (phospho Ser474) Antibody (A7006) - Antibodies.comEnlarge Image

Western Blot - Anti-Akt2 (phospho Ser474) Antibody (A94466)

Western blot analysis of 293 cells using Anti-Akt2 (phospho Ser474) Antibody.

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Proteins predicted to interact with AKT2

Predicted protein interactions based upon String database. Revelancy score correlates with probability of interaction.
99.4% Relevancy Score
99.4% Relevancy Score
99.1% Relevancy Score
98.6% Relevancy Score
98.6% Relevancy Score
98.4% Relevancy Score
98.3% Relevancy Score
98% Relevancy Score
97.9% Relevancy Score