Recombinant Anti-Biotin Antibody [BTN/8672R] (A314514)

Anti-Biotin Antibody [BTN/8672R]

Recombinant rabbit monoclonal [BTN/8672R] antibody to Biotin.

It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This MAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.

Flow Cytometry, IF, WB, IHC-P

Biotinylated proteins in solution or on tissues

In situ Nucleic Acid Hybridization Flow Cytometry: 1-2 µg/million cells, IF: 1-2 µg/ml, WB: 1-2 µg/ml, IHC: 1-2 µg/ml

Species Independent

Biotinylated sheep immunoglobulin.

Rabbit

Monoclonal

BTN/8672R

kappa

Protein A chromatography.

200 µg/ml

244Da

Liquid

Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.