Anti-BrdU Antibody (A283414)

Anti-BrdU Antibody

Rabbit polyclonal antibody to BrdU.

This antibody recognises bromodeoxyuridine (known as BrdU or BrdUrd). BrdU is a synthetic thymidine analog, which when added to cell culture media can be incorporated into the DNA of replicating cells as a substitute for thymidine. This characteristic combined with the ease of detecting incorporated BrdU with the help of anti-BrdU antibodies has resulted in establishing BrdU as a key tool for the visualization of cells in the S-Phase of the cell cycle. BrdU is also commonly used in vivo for monitoring cell proliferation in model systems; BrdU can be administered via intraperitoneal injection, subcutaneous osmotic pump implants or in drinking water.

IHC-P, IF, Flow Cytometry, ICC

FC 1: 1:50 - 1:100, ICC: 1:500, IF 2: 1:100 - 1:500, IHC-P: 1:100 - 1:500

Species Independent

Bromodeoxyuridine conjugated to Keyhole Limpet Haemocyanin.

Rabbit

Polyclonal

IgG

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline with <0.1% Sodium Azide.

Store undiluted at -20°C only. Storage in frost free freezers is not recommended. Avoid freeze-thaw cycles. Should this product contain a precipitate we recommend microcentrifugation before use.

Rabbit anti BrdU antibody recognizes bromodeoxyuridine (known as BrdU or BrdUrd). BrdU is a synthetic thymidine analog, which when added to cell culture media can be incorporated into the DNA of replicating cells as a substitute for thymidine. This characteristic combined with the ease of detecting incorporated BrdU with the help of anti-BrdU antibodies has resulted in establishing BrdU as a key tool for the visualization of cells in the S-Phase of the cell cycle. BrdU is also commonly used in vivo for monitoring cell proliferation in model systems; BrdU can be administered via intraperitoneal injection, subcutaneous osmotic pump implants (Tesfaiqzi et al. 2004) or in drinking water (Moser et al. 2004).