Western Blot - Anti-CDK1 (phospho Thr14) Antibody (A16399)
Western blot analysis of HeLa, using Anti-CDK1 (phospho Thr14) Antibody (A16399) at 1:500 dilution. HeLa cells were treated by Hydroxyurea (4 mM) at 37°C for 20 hours. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3s.
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