Rabbit polyclonal antibody to CDK1 / CDC2 (Phospho-Thr14)
Specificity
The antibody detects endogenous level of CDK1 / CDC2 only when phosphorylated at threonine 14.
Applications
WB
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around phospho rylation site of threonine14(E-G-T(p)-Y-G) derived from Human CDK1 / CDC2 .
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Formulation
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage
Store at -20˚C / 1 year
Synonyms
CDC2, CDC28A, CDKN1, Cell division control protein 2 homolog, Cell division protein kinase 1, Cyclin-dependent kinase 1, p34 protein kinase, P34CDC2
Western blot analysis of extracts from HepG2 cells treated with Forskolin using Phospho-CDK1/CDC2 (Thr14) antibody #11997.The lane on the right is treated with the antigen-specific peptide.
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