Mouse monoclonal [BA-17] antibody to Cytokeratin 19.
Specificity
This antibody reacts with cytokeratin 19 (40 kDa) in human tissue. Cytokeratin 19 is not expressed in hepatocytes; it is often co-expressed with cytokeratin 7.
Immunohistochemistry staining (paraffin sections) of cytokeratin 19 in human liver using Anti-Cytokeratin 19 Antibody [BA-17] (A86512), (diluted 1:100), detected with GAM IgG-Alexa Fluor®488 (diluted 1:200; green), cell nuclei stained with PI (1µg/ml; orange).
Separation of MCF-7 cells (red-filled) from human leukocytes (black-dashed) in flow cytometry analysis (intracellular staining) of peripheral whole blood spiked with MCF-7 cells stained with Anti-Cytokeratin 19 Antibody [BA-17] (A86512), (concentration in sample 3 µg/ml, GAM APC).
Western blotting analysis of human cytokeratin 19 using Anti-Cytokeratin 19 Antibody [BA-17] (A86512) on lysates of HT-29 cell line and MOLT-4 cell line (cytokeratin non-expressing cell line; negative control) under non-reducing and reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-cytokeratin 19 monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. A specific band was detected for cytokeratin 19 at approximately 41 kDa.
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