Mouse monoclonal [ERBB2/2452] antibody to ErbB2 (Biotin).
Applications
Flow Cytometry, IF, IHC, IHC-P
Positive Control
SKBR-3 cells or breast cancer tissue.
Dilutions
IHC-P: 1-2 µg/mL for 30 minutes at room temperature. For IHC-P, heat-induced epitope retrieval is recommended using 10 mM citrate buffer, pH 6.0, for 10-20 minutes, followed by cooling to room temperature for 20 minutes. Directly conjugated primary antibodies may require higher working concentrations than indirect detection systems.
Reactivity
Human
Immunogen
Recombinant human ErbB2 (HER2) protein fragment corresponding to approximately amino acids 311-462 (exact sequence is proprietary).
Host
Mouse
Clonality
Monoclonal
Clone ID
ERBB2/2452
Isotype
IgG2a
Light Chains
kappa
Conjugate
Biotin
Concentration
0.1 mg/mL
Predicted MW
185 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.1% BSA and 0.05% Sodium Azide.
Storage
Shipped and stored at +4°C. This product is also photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. This CF® conjugated antibody is guaranteed for 2 years from data of receipt when stored correctly.
The protein microarray results shown are representative of the unconjugated form of the Anti-ErbB2 Antibody [ERBB2/2452] (Biotin). Protein microarray analysis of more than 19,000 full-length human proteins was performed using the Anti-ErbB2 Antibody [ERBB2/2452] (Biotin). The Z-score reflects antibody binding intensity to each protein on the HuProt™ array and is expressed as the number of standard deviations above the mean array signal. The S-score represents the difference in Z-score between adjacent ranked proteins and provides a measure of target specificity. Antibodies with an S-score of at least 2.5 are considered highly specific for their intended target.
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