Mouse monoclonal [HRB2/451] antibody to ErbB2 (Biotin).
Applications
Flow Cytometry, IF
Positive Control
SKBR-3 cells or breast cancer tissue.
Dilutions
Flow Cytometry: 0.5-1 µg/million cells in 100 µL, IF: 1-2 µg/mL, IHC-P: 0.5-1 µg/mL. For IHC-P, heat-induced epitope retrieval is recommended using 10 mM citrate buffer, pH 6.0, for 10-20 minutes, followed by cooling to room temperature for 20 minutes. Directly conjugated primary antibodies may require higher working concentrations than indirect detection systems.
Reactivity
Human
Immunogen
Recombinant human ErbB2 (HER2) protein.
Host
Mouse
Clonality
Monoclonal
Clone ID
HRB2/451
Isotype
IgG1
Light Chains
kappa
Conjugate
Biotin
Concentration
0.1 mg/mL
Predicted MW
185 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.1% BSA and 0.05% Sodium Azide.
Storage
Shipped and stored at +4°C. This product is also photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. This CF® conjugated antibody is guaranteed for 2 years from data of receipt when stored correctly.
Immunofluorescence of methanol-fixed MCF7 cells stained with Anti-ErbB2 Antibody [HRB2/451] (CF®488A) (green). Nuclei were counterstained with RedDot™2 (magenta).
The protein microarray results shown are representative of the unconjugated form of the Anti-ErbB2 Antibody [HRB2/451] (Biotin). Protein microarray analysis of more than 19,000 full-length human proteins was performed using the Anti-ErbB2 Antibody [HRB2/451] (Biotin). The Z-score reflects antibody binding intensity to each protein on the HuProt™ array and is expressed as the number of standard deviations above the mean array signal. The S-score represents the difference in Z-score between adjacent ranked proteins and provides a measure of target specificity. Antibodies with an S-score of at least 2.5 are considered highly specific for their intended target.
![Immunofluorescence - Anti-ErbB2 Antibody [HRB2/451] (Biotin) (A352531) - Antibodies.com](https://cdn.antibodies.com/image/catalog/352/A352531_1.jpg?profile=product_top)
![Protein Array - Anti-ErbB2 Antibody [HRB2/451] (Biotin) (A352531) - Antibodies.com](https://cdn.antibodies.com/image/catalog/352/A352531_2.jpeg?profile=product_top)
![SDS-PAGE - Anti-ErbB2 Antibody [HRB2/451] (Biotin) (A352531) - Antibodies.com](https://cdn.antibodies.com/image/catalog/352/A352531_3.jpeg?profile=product_top)
![Immunofluorescence - Anti-ErbB2 Antibody [HRB2/451] (Biotin) (A352531) - Antibodies.com](https://cdn.antibodies.com/image/catalog/352/A352531_1.jpg?profile=product_top_thumb)
![Protein Array - Anti-ErbB2 Antibody [HRB2/451] (Biotin) (A352531) - Antibodies.com](https://cdn.antibodies.com/image/catalog/352/A352531_2.jpeg?profile=product_top_thumb)
![SDS-PAGE - Anti-ErbB2 Antibody [HRB2/451] (Biotin) (A352531) - Antibodies.com](https://cdn.antibodies.com/image/catalog/352/A352531_3.jpeg?profile=product_top_thumb)
![Immunofluorescence - Anti-ErbB2 Antibody [HRB2/451] (Biotin) (A352531) - Antibodies.com](https://cdn.antibodies.com/image/catalog/352/A352531_1.jpg?profile=product_image)
![Protein Array - Anti-ErbB2 Antibody [HRB2/451] (Biotin) (A352531) - Antibodies.com](https://cdn.antibodies.com/image/catalog/352/A352531_2.jpeg?profile=product_image)
![SDS-PAGE - Anti-ErbB2 Antibody [HRB2/451] (Biotin) (A352531) - Antibodies.com](https://cdn.antibodies.com/image/catalog/352/A352531_3.jpeg?profile=product_image)
![Immunohistochemistry (formalin-fixed, paraffin-embedded) - Anti-ErbB2 Antibody [ERBB2/2452] (Biotin) (A353972) - Antibodies.com](https://cdn.antibodies.com/image/catalog/353/A353972_1.jpeg?profile=product_alternative)
![SDS-PAGE - Anti-ErbB2 Antibody [HRB2/282] (Biotin) (A352368) - Antibodies.com](https://cdn.antibodies.com/image/catalog/352/A352368_1.jpeg?profile=product_alternative)
![SDS-PAGE - Anti-ErbB2 Antibody [HRB2/718] (Biotin) (A352737) - Antibodies.com](https://cdn.antibodies.com/image/catalog/352/A352737_1.jpeg?profile=product_alternative)
![Immunohistochemistry (formalin-fixed, paraffin-embedded) - Anti-ErbB2 Antibody [ERBB2/3080] (Biotin) (A354222) - Antibodies.com](https://cdn.antibodies.com/image/catalog/354/A354222_1.jpeg?profile=product_alternative)
![Immunohistochemistry (formalin-fixed, paraffin-embedded) - Anti-ErbB2 Antibody [ERBB2/3092] (Biotin) (A354230) - Antibodies.com](https://cdn.antibodies.com/image/catalog/354/A354230_1.jpeg?profile=product_alternative)
![Immunohistochemistry (formalin-fixed, paraffin-embedded) - Anti-ErbB2 Antibody [ERBB2/3093] (Biotin) (A354238) - Antibodies.com](https://cdn.antibodies.com/image/catalog/354/A354238_1.jpeg?profile=product_alternative)
![Immunohistochemistry (formalin-fixed, paraffin-embedded) - Anti-ErbB2 Antibody [ERB2/776] (Biotin) (A352845) - Antibodies.com](https://cdn.antibodies.com/image/catalog/352/A352845_1.jpg?profile=product_alternative)
![Immunohistochemistry (formalin-fixed, paraffin-embedded) - Anti-ErbB2 Antibody [ERBB2/3078] (Biotin) (A354206) - Antibodies.com](https://cdn.antibodies.com/image/catalog/354/A354206_1.jpeg?profile=product_alternative)
![Immunohistochemistry (formalin-fixed, paraffin-embedded) - Anti-ErbB2 Antibody [ERBB2/3079] (Biotin) (A354214) - Antibodies.com](https://cdn.antibodies.com/image/catalog/354/A354214_1.jpeg?profile=product_alternative)